Hi, This answer is originally from "Bat Call Sound Analysis Workshop" group on Facebook. The solution is to download a pre 5.4 version of "I ran into this a bit back and contacted Wildlife Acoustics. I was told that some features of Kaleidoscope version 5.4.0 did not support Audiomoth file formats except with a paid licence. They suggested I use a previous version unless I needed the features of the full paid Kaleidoscope. I use the free version 5.3.0 with Audiomoth files and it works fine for me with batch processing. Last time I looked the earlier versions are available for download from the WA website."